Fig 4. Effects of M250K and R16H/M250K mutations on PKR stability in infected cells.

HeLa cells were infected with MP-12 or mutant viruses at an m.o.i. of 3. Whole-cell lysate was harvested at 8 h p.i. and subjected to Western blot analysis by using anti-PKR antibody, anti-MP-12 antibody and anti-β-actin antibody as the primary antibody for the top, middle and bottom panels, respectively.