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. 2016 Oct 6;11(10):e0164270. doi: 10.1371/journal.pone.0164270

Fig 3. SPRF tube during re-warming under a stereomicroscope.

Fig 3

After SPRF, an aluminum tube filled with EAFS medium was cut open under liquid nitrogen and observed under a stereomicroscope during re-warming. Shown are images before ice expansion (A), and 130 ms (B), 195 ms (C), 260 ms (D), 520 ms, and (E) 1495 ms after first visible expansion occurs (see also S1 Video). Ice expands out of the tube (white arrowheads in B and C) and melts subsequently (white arrowheads D-F). Afterwards liquid drops out of the tube (black arrowheads in E and F).