Table 1. Comparison of cardiac function markers in C57 (C) and P448Lneo- (P) mutant mice at 6–8 weeks, 6 months, 8 months, 10 months and 12 months.
| 6wk | 6mo | 8mo | 10mo | 12mo | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| P | C | P | C | P | C | P | C | P | C | |
| Dias Length (cm) | 0.478±0.04 | 0.595±0.05 | 0.618±0.07 | 0.780±0.05 | 0.668±0.1 | 0.765±0.07 | (0.691±0.05) | (0.722±0.07) | (0.713±0.05) | (0.751±0.05 |
| Sys Length (cm) | 0.369±0.04 | 0.494±0.06 | 0.501±0.08 | 0.689±0.08 | 0.558±0.1 | 0.649±0.06 | 0.562±0.06 | 0.620±0.06 | (0.609±0.06) | (0.603±0.06) |
| EDV (A2C) (mL) | (0.02±0.005) | (0.04±0.01) | 0.033±0.01 | 0.66±0.03 | 0.047±0.02 | 0.076±0.02 | 0.046±0.01 | 0.072±0.02 | 0.041±0.0006 | 0.066±0.008 |
| ESV (A2C) (mL) | (0.006±0.002) | (0.018±0.008) | 0.013±0.004 | 0.031±0.02 | 0.020±0.008 | 0.033±0.01 | 0.021±0.009 | 0.037±0.01 | 0.016±0.0003 | 0.031±0.004 |
| Endo Area (cm2) | (0.112±0.02) | (0.17±0.03) | 0.158±0.03 | 0.25±0.05 | 0.198±0.04 | 0.259±0.05 | 0.198±0.03 | 0.253±0.05 | 0.190±0.02 | 0.242±0.04 |
| Epi Area (cm2) | (0.239±0.02) | (0.312±0.03) | 0.333±0.04 | 0.418±0.06 | 0.369±0.05 | 0.432±0.05 | 0.376±0.06 | 0.411±0.06 | 0.355±0.02 | 0.41±0.03 |
| Endo Length (cm) | 0.502±0.04 | 0.609±0.05 | 0.636±0.07 | 0.787±0.05 | 0.682±0.1 | 0.768±0.07 | (0.704±0.05) | (0.728±0.07) | (0.713±0.04) | (0.75±0.05) |
| EF (%) | 71.7±3.6 | 55.6±6.4 | 54.5±4.9 | 62.3±7.1 | (57.5±6.7) | (56.9±8.8) | (49.2±4.6) | (54.9±9.1) | (52.0±1.7) | (53.5±0.4) |
| SV (mL) | 0.014±0.001 | 0.022±0.006 | 0.021±0.006 | 0.035±0.01 | 0.027±0.009 | 0.45±0.009 | 0.025±0.007 | 0.035±0.009 | (0.027±0.009) | (0.035±0.008) |
| Myo-thickness (cm) | (0.087±0.003) | (0.083±0.003) | 0.102±0.003 | 0.084±0.003 | 0.092±0.002 | 0.062±0.003 | 0.095±0.005 | 0.078±0.002 | (0.093±0.003) | (0.084±0.004) |
| CO (mL/min) | 9.59±1.4 | 8.68±1.9 | 10.95±1.8 | 16.22±2.5 | 12±1.5 | 19.03±1.6 | 10.8±1.4 | 15.8±1.4 | 11.27±1.4 | 15.73±1.1 |
| Weight | 17.5 | 21.5 | 33.23 | 40.89 | 35.36 | 43.9 | 37.67 | 46.45 | 37.25 | 48.25 |
| CI (CO/Weight) | 0.54 | 0.40 | 0.33 | 0.40 | 0.34 | 0.43 | 0.29 | 0.34 | 0.30 | 0.33 |
At 6 weeks of age significant differences were demonstrated in the length of the heart as well as the ejection fraction and stroke volume. However, by 6 months of age significant differences are noted among all of the cardiac function markers. The ejection fraction (EF) of the P448Lneo- mice begins to normalize at 8 months of age with the length of the heart nearing normal at 10 months. By 12 months of age the systolic (ESV) and diastolic (EDV) volumes as well as the endocardial and epicardial area remain significantly different. Cardiac output (CO) remains significantly lower beginning at 6 months of age through 12 months. The weight of the P448Lneo- mouse remains smaller throughout the 12 month period. The cardiac index (CI) is higher in the 6 week old P448Lneo- have a higher cardiac index however it becomes smaller by 6 months of age and remains lower than the C57 control throughout the 12 month time period. Statistical significance determined through students t-test (p<0.05). Non-statistically significant difference noted bold, italics and parentheses. N of at least 5 for each time point. Data represents a longitudinal study of the same animals throughout a 12 month period. Some animals were sacrificed at each time point for histological analysis.