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. 2016 Oct 6;11(10):e0164146. doi: 10.1371/journal.pone.0164146

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© 2016 Kumar et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A549 cells were transfected with siRNA-NCL or siRNA-NT followed by infections. Infected cells were fixed at indicated time points and subjected to double IFA staining using anti-nucleolin, anti-viral NP antibodies and secondary antibodies with two fluorophores. [a] Reduced expression of endogenous nucleolin in cells transfected with siRNA-NCL in comparison with the control construct siRNA-NT [b] Disruption of nucleolin and NP interaction in cells transfected with siRNA-NCL and co-localization of two proteins in cells with siRNA-NT at 6hrs post infection.