Table 1. Primers used in the study.

Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and underlined.

Primer name	Sequence 5’ to 3’	Restriction enzyme site
Mut IHNV-KpnI	GACCCATCCAGATGTTTACGGTACCCTGACTCTTAGATAGAAAAAAATG	KpnI
IHNV-G-SP-NheI-F	CTGATCACCTGCGGAGCAGCTAGCCAAACCGCCAAACCCG	NheI
IHNV-TM-Pfl23II-R	ATTCGACCTTCAATCACGTACGTGGAGTTTCTGGCCCACAA	Pfl23II
VHSV-TM-Eco72I-R	CCCACAACCCCCATCCCAGACACGTGACCGCTGAACAAAACTCC	Eco72I
HA-SP-NheI-F	AGAAATCGTGCTAGCHACACAATATGTATAGGCTACC	NheI
HA-TM-Eco72I-R	TCTATTACGCACGTGAATCTGATAGATCCCCATTGATTCC	Eco72I
HA-TM-Pfl23II-R	TCTATTACGCGTACGAATCTGATAGATCCCCATTGATTCC	Pfl23II
GS-G-IHNV F2	ATAAATCGTGGTACCCTGACTCTTAGATAGAAAAAAATGGCACTATAGT	KpnI
GS-G-IHNV F1	GGCACTATAGTGCTTTTCAACTACTAGTATGGACACCACGATCACC	SpeI
GE-G-IHNV R2	ATTACGGGTACCGTAAACATCTGGATGGGTCGAGGGGCGGGAGAGGAAAAAGGAGGGGG	KpnI
GE-G-IHNV R1	GAGGAAAAAGGAGGGCGGGGCTGTTTGTGTTACGTATTAGGACCGGTTT	SnaBI