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. 2016 Oct 6;12(10):e1006353. doi: 10.1371/journal.pgen.1006353

Fig 1. A tristable white-hybrid-opaque switch operates in C. tropicalis.

Fig 1

(A) A white colony of MTLa/a strain CAY1504 was grown on a Spider plate at 22°C for 10 days. The white (w) colony sectored to hybrid (h, light gray arrowhead) and to opaque (o, dark gray arrowhead). Representative cellular morphologies of (B) white cells, (C) hybrid cells, and (D) opaque cells derived from strain CAY1505. Scale bars = 10 μm. (E, F) Switching frequencies between the three phenotypic states. (E) MTLa/a and (F) MTLα/α strains were grown on Spider medium at 22°C for 10 days and switching frequencies determined. (G) Mating frequencies of cells from white (W), opaque (O), and hybrid (H) phenotypes. Mating experiments were performed for 1 day at 22°C on Spider medium. Error bars show standard error, with each data point representing at least 9 replicate experiments. Marked data points (*) are significantly higher than unmarked data points (p<0.001, two-tailed T-test). (H) Growth rates for the three phenotypic states. The average doubling time during logarithmic phase growth was determined for white, hybrid, and opaque cells in YPD and Spider media and plotted with standard deviations.