Fig 6. Sugar chains of OmpA-like protein contribute to lectin binding.

The wells of 96-well microtiter plates were coated with 5 μg/ml OmpA-like protein for 18–24 h. After blocking, 200 mM GlcNAc, 200 mM NeuAc, or 10 mM mannose was added to the wells for 2 h before addition of 10 μg/ml Fc-conjugated recombinant proteins for 3 h. Binding was determined by an Fc-specific ELISA. The results are expressed as the mean ± SD (n = 3). *, P < 0.01.