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. 2016 May 31;15(19):2585–2592. doi: 10.1080/15384101.2016.1190892

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Figure 5. — siRNA-mediated targeting of HMGI-C strongly sensitized MDA-MB-468 cells by stimulation of apoptosis. Untreated (A), and 80 pmol HMGI-C treated MDA-MB-468 (B) were prepared and assayed with TUNEL assay. FACS analysis of cell distribution for apoptosis and necrosis on MDA-MB-468 cells for untreated (C), (NC) siRNA (D) and 80 pmol siRNA (E), prepared after 48 h. Percentage of dual-positive (Annexin V and PI positive) cells from 3 independent experiments were quantified and presented as mean ± SD (n = 3). (F) Relative P53, caspase3, 9, 8, Bcl2 (5G, 5H) protein expression were evaluated by immune-blotting and densitometry using imageJ software. *p < 0.05, ****p < 0.0001, vs. control group.