Figure 1.

USP1 or UAF1 depletion inhibits HR repair by primarily affecting downstream of RAD51 foci formation. A. USP1 and FANCD2 are not epistatic in the DR-GFP assay. siRNAs are transfected to the U2OS-based HR reporter cells, followed by I-Sce1 transfection. 48 hours later, cells were harvested for Flow cytometric analysis. Three independent experiments were performed (*p<0.001). B, C. UAF1 (B) or USP1 (C) knockdown impairs the disappearance of the RAD51 foci. HeLa cells were treated with UV (20 J/m²), then the cells were fixed at the corresponding time points for IF procedures. On the right are the representative images.