Figure 3.

The PKH^Bright daughter stem cells exhibit a higher capability of forming tumorispheres and colonies. (A) Flow cytometry results for showing the gated regions of PKH^Bright (R1) and PKH^Dim (R2) of serum-differentiated HT29 SDCSCs before sorting and the purities after sorting. The percentage of cells in the gated region is illustrated in the corresponding panels. (B) Representative images of spheroids formed from HT29 SDCSCs- and HCT15 SDCSCs-derived PKH^Bright and PKH^Dim daughter cells. Scale bar = 100μm. (C) Histograms for showing the secondary sphere formation capacities of HT29 SDCSCs- and HCT15 SDCSCs-derived PKH^Bright and PKH^Dim daughter cells. Bright, PKH^Bright cells; Dim, PKH^Dim cells. Data represent mean ± SD n = 3 independent experiments. ***, p < 0.001 (Student's t test). (D) Histograms for showing the anchorage-independent colony forming capacities of HT29 SDCSCs- and HCT15 SDCSCs-derived PKH^Bright and PKH^Dim daughter cells. LPF, low power field. *, p < 0.05 (Student's t test).