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. 2016 Aug 11;15(20):2697–2703. doi: 10.1080/15384101.2016.1218101

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© 2016 Taylor & Francis

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Figure 4. — Restoration of Numb directs the asymmetry of PKH26 vesicles. (A) Histograms for showing percentages of PKH26 vesicle asymmetry/symmetry in the indicated stable cell lines. PKH-Asym, asymmetric segregation of PKH26 vesicles. n (total counted cells over 2 independent experiments) = 110, 34, 75, 123, 145, 137, 137, and 138 for HT29 SDCSC-Vec, HT29 SDCSCs-FLAG-Numb(162-400), HT29 SDCSCs-Numb(FL) and HT29 SDCSCs-Numb(FL)/β-cat(mut), HCT15 SDCSC-Vec, HCT15 SDCSCs-FLAG-Numb(162-400), HCT15 SDCSCs-Numb(FL) and HCT15 SDCSCs-Numb(FL)/β-cat(mut), respectively. The p-value is estimated by χ² test. *, p < 0.05; ***, p < 0.001. (B) Histograms for showing percentages of PKH26 vesicle asymmetry/symmetry at indicated stable cell lines. n (total counted cells over 2 independent experiments) = 146, 148, 143, 147, 106, 75, 115 and 119 for HT29-Vec, HT29-146a, HT29-Puro-Vec, HT29-146a/Puro-Numb, HCT15-Vec, HCT15-146a, HCT15-Puro-Vec and HCT15-146a/Puro-Numb, respectively. The p-value is estimated by χ² test. *, p < 0.05; **, p < 0.01. (C) Upper, RT-qPCR result for showing the expression of miR-146a-5p in HCT15 SDCSC-Vec and HCT15 SDCSC-Numb(FL) stable lines. Lower, RT-qPCR result for showing the expression of miR-146a-5p in PKH^Bright and PKH^Dim daughter cells of HCT15 SDCSCs-Numb(FL) cultured in stem-cell medium. PKH^Bright cells; Dim, PKH^Dim cells. *, p < 0.05; **, p < 0.01 (Student's t test). (D) A schema for summarizing the impact of Numb in switching division modes of colorectal cancer stem cells.