Figure 5.

Compound 6j prevents BLM-induced lung fibrosis. Fourteen days after the onset of BLM injury, C57BL/6 mice (five animals each group) received an oral gavage of 6j twice daily at the indicated dosages, and the lungs were collected on day 28. The upper panels in (A) display the hematoxylin and eosin (H&E) staining images of the dissected lungs. The bottom panels in (A) represent Masson’s trichrome staining. The arrows indicate the fibrotic areas of the tissues. The images in (B) show the results of the immunoblotting with the indicated antibodies. The histogram in (C) shows the determined hydroxyproline content. ***P < 0.001.