Figure 4.

Effect of SU1498 on VEGF-induced ADAM-33 expression and cell proliferation in ASM cells. ASM cells were incubated with indicated doses of SU1498 for 2 h before treatment with VEGF (50 ng/ml) for 24 h, and then western blotting analysis for ADAM-33 was performed. β-actin was used as a loading control (A). ASM cells were incubated with indicated doses of SU1498 for 2 h before treatment with VEGF (50 ng/ml) for 48 or 72 h, and then cell proliferation was determined by BrdU incorporation (B). All experiments were done at least twice. Values represent the means ± SEM. *P < 0.05 vs. control; ^# P < 0.05 vs. VEGF alone.