Figure 5.

Effect of VEGF and SU1498 on phosphorylation of ERK1/2 and Akt in ASM cells. ASM cells were incubated at indicated times of VEGF (50 ng/ml), and then western blotting analysis for phospho-ERK 1/2 (A) and phospho-Akt (B) was performed. ASM cells were incubated with 0.5µM SU1498 for 2 h before treatment with VEGF (50 ng/ml) for 15 min, and then western blotting analysis for phospho-ERK 1/2 was performed (C). ASM cells were incubated with 0.5µM SU1498 for 2 h before treatment with VEGF (50 ng/ml) for 30 min, and then western blotting analysis for phospho-Akt was performed (D). The total ERK1/2 and Akt was used as a loading control. All experiments were done at least twice.