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. 2016 Jul 19;15(19):2608–2618. doi: 10.1080/15384101.2016.1208869

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© 2016 Taylor & Francis

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Figure 3. — Knockdown of Stau2 impacted the attachment between microtubules and kinetochores. After morpholino treatment, MI oocytes were incubated on ice for 5 min and then processed with immunofluorescent staining using CREST serum and acetylated-tubulin antibody. The attachment between the microtubules and kinetochores was carefully analyzed. The microtubule-kinetochore attachment was frequently disrupted in oocytes treated with Stau2 morpholino oligo (A, h: arrows). Further statistical analysis demonstrated that the percentage of oocytes with abnormal microtubule-kinetochore attachment was significantly higher in Stau2 morpholino group than that in 2 control groups (P < 0.05) (B). Microtubules were visualized in red, CREST was visualized in green and DNA was labeled in blue. Scale bar = 20 μm.