Figure 4.

Stau2 depletion delayed meiosis I completion in oocytes. After morpholino treatment, oocytes were cultured in normal maturation medium for 17 h, and processed for immunostaining with acetylated-tubulin and Stau2. The meiotic progression was counted based on chromosome configuration and spindle structure. The percentage of oocytes at MI stage was significantly higher in Stau2 morpholino group, quite some oocytes were at the late stage of TI (A, e-h, B). Only faint Stau2 was detected with immunofluorescence on microtubules in TI oocytes (A, f, g, h), combined with decondensed chromosomes (A, e).