Figure 3.

Tax effect on the physical association of E2-ERα with other transcriptional cofactors. MCF-7 cells were or not transfected with 1.5 μg of w.t.Tax expressing plasmid. The indicated cells were treated with E2 at 5 hr before extracting the cells for coimmunoprecipitation (co-IP) analyses. The whole cell extracts were immunoprecipitated with ERα mouse specific monoclonal antibody as indicated in the figure. The various immunoprecipites were analyzed by Western blot analysis with ERα, Ahr, 53Bp1, CBP, Src and Tax rabbit specific monoclonal antibodies.