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Effect of various co-factors silencing on BRCA1 and pS2 expression. MCF-7 cells were transfected with the shRNAs of the indicated cofactors and treated with E2 w 5 hr before extracting the cells for western blot analysis. The whole cell extracts of the cells were examined for BRACA1 and pS2 expression by western blot analysis with anti BRCA1 and pS2 monoclonal antibodies.
