Figure 4.

MED26 recruitment by MLL fusion proteins is dispensable for myeloid transformation. (A) Transforming ability of the MTM fusion constructs with various subdomains of the pSER domain. A schematic of the myeloid progenitor transformation assay is shown on top. Hoxa9 expression in the first round of colonies normalized to Gapdh expression is shown as the relative value of MTM-AF4-2C-bc (arbitrarily set at 100%) with error bars (SD of PCR triplicates). The number of colony-forming units (CFUs) of third and fourth rounds is shown with error bars (SD was calculated from data arising from more than 3 independent experiments). (B) Protein expression of various MTM fusion proteins in virus packaging cells. The whole cell extracts of the virus-packaging cells were analyzed by WB using anti-HA antibody.