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. 2016 Apr 7;7(19):28329–28339. doi: 10.18632/oncotarget.8631

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Copyright: © 2016 Debeb et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A and B) Flow cytometric analysis of ROS with 2′-7′-dichlorofluorescein diacetate (DCF-DA). ROS concentrations were higher in ALDH⁻ (A) and ALDH⁺ (B) cells treated with VA versus vehicle-treated controls. (C and D) Flow cytometric analysis of mitochondrial superoxide with MitoSOX Red. Superoxide levels were higher in VA-induced HDACi-CSCs from ALDH⁻ (C) and ALDH⁺ (D) cells than in vehicle-treated differentiated cells. Representative overlaying merged graphs are shown with bar graphs summarizing quantification of the DCFDA and Mitosox-red data from several experiments.