Fig 5. Changes to the lifespan and autophagy profiles of chico¹/+ male flies.

(A) The average lifespan profiles of chico¹/+ mutant male flies exposed to ad libitum or IF treatment conditions, starting at 1-week of age. (B) Total head protein extracts from control (0h) or fasted (4h) male flies at 1-week, 3-week or IF-treated 3-week of age (n = 3), were used for Western blot analysis of the Atg8a, Ref(2)P, and Actin proteins. (C) The relative ratio of Atg8a-II to Atg8a-I proteins. *P ≤ 0.05.