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. 2016 Oct 6;11(10):e0164037. doi: 10.1371/journal.pone.0164037

Table 1. Labeled Purkinje cell axonal boutons after FL lentivirus injections.

Case Injection site Boutons in MVN Other vestibular nuclei
MVMC MVPC PrH
6R whole FL, >10 neurons in PFL +++ ++++ ++++ LV, SuV, y
12L whole FL, a few neurons in PFL +++ ++++ +++ LV, SuV, y
14R FL (zones 2 and 3), a few neurons in PFL ++ ++ + LV, SuV
14L whole FL, a few neurons in PFL +++ ++++ +++ LV, SuV, LatPC
15R whole FL +++ +++ ++ LV, SuV, LatPC
16L FL (zones 1, 2, and 3) + +++ +++ LV, SuV, y
24R whole FL, a few neurons in PFL ++ +++ ++++ LV, SuV, y
71L FL (zones 2, 3, and 4), a few neurons in PFL ++++ ++ +++ LV, SuV
74R whole FL +++ ++++ ++++ LV, SuV, y
82R whole FL, >10 neurons in PFL ++ +++ +++ LV, SuV
94R whole FL, a few neurons in PFL ++++ ++++ ++++ LV, SuV
94L whole FL, >10 neurons in ventral PFL +++ ++++ ++++ LV, SuV
95R FL (zones 2, 3, and 4), a few neurons in PFL ++++ +++ + LV, SuV

Density of labeled boutons for each subregion were evaluated for two brain sections for each case (approximately 6.00 mm and -6.36 mm from bregma) as +, 0–50 boutons; ++, 50–100 boutons; +++, 100–200 boutons; ++++, >200 boutons. FL, flocculus; LatPC, parvocellular region of lateral cerebellar nucleus; LV, lateral vestibular nucleus; MVN, medial vestibular nucleus; MVMC, magnocellular MVN; MVPC, parvocellular MVN; PFL, paraflocculus; PrH, prepositus hypoglossi nucleus; SuV, superior vestibular nucleus; y, group y. Injection sites in FL are shown as zones 1~4, according to [12], in which FL was divided to four sagittal zones in the lateral-ventral to medial-dorsal direction. Labeled neurons in PFL were counted at the level of the injection site.