Table 1. Labeled Purkinje cell axonal boutons after FL lentivirus injections.
Case | Injection site | Boutons in MVN | Other vestibular nuclei | ||
---|---|---|---|---|---|
MVMC | MVPC | PrH | |||
6R | whole FL, >10 neurons in PFL | +++ | ++++ | ++++ | LV, SuV, y |
12L | whole FL, a few neurons in PFL | +++ | ++++ | +++ | LV, SuV, y |
14R | FL (zones 2 and 3), a few neurons in PFL | ++ | ++ | + | LV, SuV |
14L | whole FL, a few neurons in PFL | +++ | ++++ | +++ | LV, SuV, LatPC |
15R | whole FL | +++ | +++ | ++ | LV, SuV, LatPC |
16L | FL (zones 1, 2, and 3) | + | +++ | +++ | LV, SuV, y |
24R | whole FL, a few neurons in PFL | ++ | +++ | ++++ | LV, SuV, y |
71L | FL (zones 2, 3, and 4), a few neurons in PFL | ++++ | ++ | +++ | LV, SuV |
74R | whole FL | +++ | ++++ | ++++ | LV, SuV, y |
82R | whole FL, >10 neurons in PFL | ++ | +++ | +++ | LV, SuV |
94R | whole FL, a few neurons in PFL | ++++ | ++++ | ++++ | LV, SuV |
94L | whole FL, >10 neurons in ventral PFL | +++ | ++++ | ++++ | LV, SuV |
95R | FL (zones 2, 3, and 4), a few neurons in PFL | ++++ | +++ | + | LV, SuV |
Density of labeled boutons for each subregion were evaluated for two brain sections for each case (approximately 6.00 mm and -6.36 mm from bregma) as +, 0–50 boutons; ++, 50–100 boutons; +++, 100–200 boutons; ++++, >200 boutons. FL, flocculus; LatPC, parvocellular region of lateral cerebellar nucleus; LV, lateral vestibular nucleus; MVN, medial vestibular nucleus; MVMC, magnocellular MVN; MVPC, parvocellular MVN; PFL, paraflocculus; PrH, prepositus hypoglossi nucleus; SuV, superior vestibular nucleus; y, group y. Injection sites in FL are shown as zones 1~4, according to [12], in which FL was divided to four sagittal zones in the lateral-ventral to medial-dorsal direction. Labeled neurons in PFL were counted at the level of the injection site.