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. 2016 Oct 6;11(10):e0164285. doi: 10.1371/journal.pone.0164285

Fig 2. HSPB1 sustains cellular NADPH and pentose levels.

Fig 2

(A) NADPH/NADP+ ratio in stable HSPB1-knockdown U87 cells was determined. (B) GSH level in stable HSPB1-knockdown U87 cellswas quantified. (C-D) ROS level in stable HSPB1-knockdown U87 cells was determined by using DCF (C). An oxidation-insensitive dye was included as a negative control (D).(E) Ribulose-5-phosphate content in stable HSPB1-knockdown U87 cells was quantified. (F) Relative RNA synthesis rate in stable HSPB1-knockdown U87 cells was determined.