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. 2016 Oct 6;11(10):e0164285. doi: 10.1371/journal.pone.0164285

Fig 4. HSPB1 activates G6PD by promoting its SIRT2 interaction and deacetylation.

Fig 4

(A) Acetylation and phosphorylation levels of immunoprecipitated endogenous G6PD from HSPB1-knockdown U87 cells were determined by western blot. (B) The activity of endogenous G6PD was determined in HSPB1-knockdown U87 cells. (C) Acetylation and phosphorylation level of immunoprecipitated endogenous G6PD from HSPB1-knockdown U373 cells was determined by western blot. (D) The activity of endogenous G6PD was determined in HSPB1-knockdown U373 cells. (E) Interaction between endogenous G6PD and SIRT2 was determined by immunoprecipitation and western blot in HSPB1-knockdown U87 cells.(F) Interaction between endogenous G6PD and SIRT2 was determined by an inverse co-immunoprecipitation and western blot in HSPB1-knockdown U87 cells.