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. 2016 Oct 6;12(10):e1005935. doi: 10.1371/journal.ppat.1005935

Fig 4. Affinity of p19 to perfect or mismatch-containing duplex si/miRNAs in vitro.

Fig 4

Band shift assay of perfect duplex siR171 (A), and mismatched miR171a (B), miR171b (C) and miR171c (D) duplex RNAs’ with p19 protein. The structure of dsRNAs is shown above the gel pictures. Direct measurement of the absolute apparent dissociation constant Kd values (F) were calculated as previously described [57] based on the quantification of band intensities of p19:dsRNA bound fraction as a function of p19 protein concentration (E). Relative dissociation constant (Krel) was calculated by normalization of Kd values to Kd of siR171 (F).