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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Thromb Haemost. 2015 Aug 13;114(6):1144–1155. doi: 10.1160/TH15-01-0079

Figure 5. Localization of painted EPCR-GPI in lipid rafts.

Figure 5

To determine the localization of painted EPCR-GPI on cells, cell membranes were fractionated on a density gradient. (A) Distribution of endogenous EPCR and painted EPCR-GPI in membrane fractions of wild type EA.hy926 cells or EA.hy926 EPCRKD cells painted with 20 μg/ml EPCR-GPI. Caveolin-1 (cav-1) was used as a marker for lipid rafts. Representative blots of three independent experiments are shown. (B) Quantification of endogenous EPCR or EPCR-GPI in caveolin-enriched lipid raft (fractions 1–8) for wild type EA.hy926 cells (EPCR) and painted EA.hy926 EPCRKD cells (EPCR-GPI). Shown are mean ± SD of three independent experiments. *** P < 0.001.