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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Thromb Haemost. 2015 Aug 13;114(6):1144–1155. doi: 10.1160/TH15-01-0079

Figure 8. Stimulation of PAR cleavage by EPCR-GPI painting.

Figure 8

To determine whether EPCR-GPI painting supports APC-mediated PAR cleavage, N-terminal cleavage of PAR by APC was analyzed on HEK-293 cells expressing SEAP-PAR1 (A) or SEAP-PAR3 (B) that were painted with 80 μg/ml EPCR-GPI (+EPCR-GPI) or left untreated (-EPCR-GPI). APC-mediated PAR cleavage was expressed as the percentage of total PAR on the cell surface. (C) Induction of Akt phosphorylation at Ser473 by APC (50 nM) at 45 min on EPCRKD EA.hy926 cells in the presence and absence of EPCR-GPI painting (20 μg/ml). Shown are mean ± SD of a representative experiment of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, ns = not significant.