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. 2016 Apr 20;7(19):26935–26948. doi: 10.18632/oncotarget.8854

Figure 1. Decreased proliferation and premature senescence of Gadd45b−/− MEFs.

Figure 1

A. Gadd45b+/+ (WT - solid black) and Gadd45b−/− (KO - solid grey) MEFs were cultured at 21% oxygen continuously for 14 passages. Cells were split every 3 days, and the total numbers of cells were counted and mean population doublings (MPD) were determined. B. Cumulative cell number is plotted against passage number. C. Gadd45b+/+ and Gadd45b−/− MEFs were stained for SA-β-gal at each passage. SA-β-gal positive cells were counted in at least 10 fields from triplicate plates. A quantification of SA-β-gal positive Gadd45b+/+ (solid black) and Gadd45b−/−(solid grey) MEFs is shown for different passages. D. Representative images of Gadd45b+/+ and Gadd45b−/− MEFs stained for SA-β-gal at passage 9.