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. 2016 Apr 20;7(19):26935–26948. doi: 10.18632/oncotarget.8854

Figure 7. Increased senescence of Gadd45b−/− mice is not restricted to tissue culture associated stress.

Figure 7

A. Gadd45b+/+ (WT - black) and Gadd45b−/− (KO - grey) MEFs cultured at 21% oxygen (passage 3) were treated with sub-lethal doses of UV-irradiation (30 J/m2) or H2O2 (150 uM). Stress-induced senescence (mean numbers of SA-β-gal -positive cells) was determined 7 days after stress. *P < 0.05. B. Photograph of SA-β-gal staining of Gadd45b+/+ and Gadd45b−/− E14 embryos. Arrows indicate embryonic regions with strong senescence staining. Strong staining was found in the head and neck regions of all mutant embryos tested (n = 6).