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. 2016 Mar 25;7(19):27142–27157. doi: 10.18632/oncotarget.8382

Figure 1. PFKP mRNA and protein is up-regulated in human ccRCC tissues.

Figure 1

A.-C. Real time PCR analysis for PFKP (A), PFKM (B) and PFKL (C) mRNA levels in human ccRCC tissue samples (T) and adjacent non-malignant kidney tissues (N) (n = 19). 18S ribosomal RNA serves as an internal control. (D) Bar graph for average percentage of PFKP, PFKM and PFKL mRNA in 18 human ccRCC tissue samples (T) and 18 adjacent non-malignant kidney tissues (N) based on absolute quantification PCR analysis. E. Western blot for protein extracts from human ccRCC tissue samples (T) and adjacent non-malignant kidney tissues (N) (n = 18). F. Quantification of western blot for PFKP, PFKM, PFKL using Gel-Pro analyzer 4. G. Immunohistochemistry analysis for ccRCC and non-malignant kidney tissue samples using either normal serum (NC) or the anti-PFKP antibody. H. Western blot for protein extracts of 293T cells transiently transfected with the control vector or plasmids containing V5-tagged PFKP, PFKM or PFKL cDNA.