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. 2016 Mar 25;7(19):27142–27157. doi: 10.18632/oncotarget.8382

Figure 2. PFKP knockdown leads to impaired cell proliferation, cell cycle arrest and apoptosis in kidney cancer cell lines.

Figure 2

A. Western blot for protein extracts of normal kidney epithelial cell line HK-2 and 3 kidney cancer cell lines including 786-O, 769-P, Caki-1. B. PFK enzymatic activity in HK-2,786-O, 769-P and Caki-1 cell extracts. C. Western blot for protein extracts of 786-O and Caki-1 cells transiently transfected with control (siCtrl) or PFKP siRNAs (siPFKP1, siPFKP2 and siPFKP3) at 72 hours after transfection. D.-E. Cell growth curves of 786-O (D) and Caki-1 (E) cells transiently transfected with siCtrl, siPFKP1 or siPFKP3. F. Cell cycle analysis for Caki-1 cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 at 72 hours after transfection based on Brdu incorporation assay. G.-H. Percentage of apoptotic cells in 769-P (G) and Caki-1 (H) cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 at 72 hours after transfection according to annexin V and propidium iodide (PI) staining. * indicates p < 0.05 in B, F, G and H.