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. 2016 Mar 25;7(19):27142–27157. doi: 10.18632/oncotarget.8382

Figure 4. FBP deficiency due to PFKP suppression activates the p53 pathway in kidney cancer cells.

Figure 4

A., C. Western blot analysis for Caki-1 (A) and 769-P (C) cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 using antibody to PFKP, p-p53, p53, p21and actin at 72 hours after transfection. B., D. Real time PCR analysis for Caki-1 (B) and 769-P (D) cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 at 72 hours after transfection. E. Western Blot analysis for Caki-1cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 after 1mM or 2mM FBP treatment at 72 hours after transfection. F. Quantification of western blot for p-p53, p53 normalized by corresponding actin levels using Gel-Pro analyzer 4. G. Cell growth curves of Caki-1cells transiently transfected with siCtrl, siPFKP1 or siPFKP3 after 1mM or 2mM FBP treatment. * indicates p < 0.05 in B and D.