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. 2016 Mar 30;7(19):27280–27294. doi: 10.18632/oncotarget.8465

Figure 5. Activation of STAT3 signaling pathway was involved in the regulation of LGR5 expression.

Figure 5

The MTT assay was performed to assess cell viability in A431 cells A. and HaCaT cells B. that were stably that were stably transfected with an inducible Tet-on IKKα shRNAs and the cells were treated with Stattic. C.. A luciferase reporter assay was carried out to evaluate LGR5 promoter activity in 293 cells after the treatment of IKKi-II and/or Stattic. All promoter luciferase intensity was normalized to the pRL renilla luciferase control reporter. * p < 0.05, ** p < 0.01. D.. ChIP analysis in HaCaT cells and chemicals treatment indicated was performed to detect p-STAT3 binding to LGR5 gene. E.. ChIP analysis in A431 cells after Tet-on shIKKα transfected and chemicals treatment indicated was performed to detect p-STAT3 binding to LGR5 promoter gene. F.. A431 (Left) and HaCaT (Right) with treatment of Stattic were examined for the expression of selected proteins by Western analysis. ** p < 0.01