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. 2016 Mar 29;7(19):27363–27378. doi: 10.18632/oncotarget.8455

Figure 4. Effects of azacytidine (AZA) and decitabine (DAC) on protein synthesis and stability.

Figure 4

(A) HCT116 cells were treated with 5 μg/mL cycloheximide (CHX), or different doses of AZA or DAC for 6 and 24 h, and protein synthesis was examined by a puromycin-incorporation assay as described in “Materials and Methods”. Upper part: ponceau S staining of nitrocellulose membranes. Lower part: Western blots of puromycin. (B) HCT116 and RKO cells were treated with 5 μg/mL CHX, 50 μM AZA, or 50 μM DAC for the indicated time intervals, and whole-cell lysates were subjected to a Western blot analysis using antibodies against c-MYC or GAPDH.