Figure 5. Effects of azacytidine (AZA) and decitabine (DAC) on the cell cycle and p53 expression.

(A) HCT116 cells were treated the different doses of AZA or DAC for 24 and 48 h, and the cell cycle was analyzed by flow cytometry as described in “Materials and Methods”. (B) HCT116 cells were treated the different doses of AZA or DAC for 24 h, and whole-cell lysates were subjected to a Western blot analysis using antibodies against p53, p53R2, γH2AX, or GAPDH.