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. 2016 Mar 28;7(19):27394–27407. doi: 10.18632/oncotarget.8423

Figure 6. Neuroendocrine-like cells promote the chemotaxis activity of TAM via CXCL10 and CXCL11.

Figure 6

A. The Neuroendocrine-like cells for chemokine family screening by RT-PCR. B. ELISA was used to confirm that the CXCL10 and CXCL11 increased obviously in the supernatant of neuroendocrine-like cell (LOVO-HA-CgA). Bars correspond to the mean ± SD. C. Western blot and RT-PCR was used to confirm that the CXCL10 and CXCL11 increased obviously in the neuroendocrine-like cell (LOVO-HA-CgA). D. Western blot was used to verify the knocking down of CgA in the LOVO cells by SiRNA. E. Western blot and RT-PCR was used to confirm that the CXCL10 and CXCL11 decreased obviously in the colon cell which was knocked down of CgA (LOVO-SiRNA-CgA). F. Transwell assay was used to compare the migration ability between LOVO-SiRNA-CgA with LOVO-control cells, and the numbers of cells passed through the Matrigel matrix. Bars correspond to the mean ± SD.