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. 2016 Mar 30;7(19):27489–27498. doi: 10.18632/oncotarget.8481

Figure 2. BKM1644 inhibits survivin expression through a Stat3-dependent mechanism.

Figure 2

(A) BKM1644 (5 μM) inhibits survivin proteins and the phosphorylation of Stat3 at Ser727 and Tyr705 residues in Western blot analysis. (B) Top panel: human survivin promoter contains two putative Stat3 binding elements; Bottom panel: BKM1644 selectively inhibits the luciferase activity of pSurvivin-Luc1430 in a dose-dependent manner. The reporters were transfected into C4-2 cells for 24 h prior to further treatment with BKM1644 for 48 h. (C) Top panel: Docetaxel treatment (2.5 nM) increases survivin protein expression in a time-dependent manner in C4-2 cells; Bottom panel: BKM1644 (5 μM) antagonizes survivin induction by docetaxel (2 nM) and activates apoptosis in C4-2 cells during a 24-h culture. (D) BKM1644 sensitizes C4-2 cells to docetaxel treatment in a dose-dependent manner (72 h).