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. 2016 Mar 30;7(19):27584–27598. doi: 10.18632/oncotarget.8488

Figure 1. LCAF impedes the differentiation and function of DCs.

Figure 1

A. The CMs of LCAF decreased the differentiation of DCs from CD14+ monocytes. The CMs of LCAF decreased IL-12 B. and increased the IL-10 C. in DCs. Reduced function of LCAF-derived DCs on T cell proliferation D. Normal human lung primary fibroblasts (NHLF) were cultured with or without CL1-5 cells for 24 h, and the condition medium was collected (NHLF-CM and CL1-5-LCAF-CM). CD14+ monocytes were cultured with various CMs (20%) containing GM-CSF (20 ng/ml) and IL-4 (20 ng/ml) for 5 days. After stimulation by IFN and LPS, mature DCs were co-cultured with naïve CD4 T cells for another 5 days. The expression of DCs' surface markers was measured by flow cytometry, and the levels of cytokines in the DC supernatants were determined by MILLIPLEX MAP kits. All results are representative of at least three independent experiments, and each value is the mean ± SD of three determinations. The results were reported as mean ± SD; *p < 0.05.