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. 2016 Mar 30;7(19):27584–27598. doi: 10.18632/oncotarget.8488

Figure 4. AKT/CREB and AKT/WNK1 pathways mediated Kyn-mediated cell proliferation and migration.

Figure 4

A. LACF-CM and Kyn activate AKT, CREB and WNK1. B. AKT is the upstream regulator of CREB and WNK. C. CREB is involved in Kyn-mediated cell proliferation. D. WNK is the major factor to increase cell migration induced by Kyn. CL1-5 and A549 cells were pretreated, with or without PI3K inhibitor (LY294004) or CREB inhibitor for 1 h, and LCAF-CMs (20%) and Kyn (50 mM) were added for another 3h. The knockdown of WNK was carried out by WNK1 shRNA transfection. Control or WNK1 shRNA transfected CL1-5 and A549 cells were committed to migration analysis, as described in Figure 1. Protein expression was assessed by immunoblot analysis. All results are representative of at least three independent experiments and each value is the mean ± SD of three determinations. The results were reported as mean ± SD; *p < 0.05.