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. 2016 Mar 25;7(19):27613–27626. doi: 10.18632/oncotarget.8376

Figure 3. The regulation of E2F3a/b on miR-200b expression and chemosensitivity of LAD cells.

Figure 3

E2F3a/b mRNA and protein expression levels in SPCA1/DTX, H1299/DTX cells and the parental SPC-A1, H1299 cells before A. and after B. manipulated E2F3a and E2F3b expressions were detected by qRT-PCR and Western Blot analysis, respectively. Abundance of mRNA and protein was normalized to U6 RNA and GAPDH, respectively. C. Quantifications of miR-200b expression in different manipulated LAD cell lines as indicated was also achieved by qRT-PCR. Abundance of miRNA was normalized to U6 RNA. Data are representative of at least three independent experiments and are shown as mean±SEM. *p < 0.05, **p < 0.01 vs. control group.