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. 2016 Mar 25;7(19):27613–27626. doi: 10.18632/oncotarget.8376

Figure 6. The in vivo effects of E2F3b on miR-200b expression and chemosensitivity of LAD cells.

Figure 6

SPC-A1 and SPC-A1/DTX cells were transfected with pSil/shE2F3 or pSil/shE2F3-NC and injected subcutaneously into nude mice. When the average tumor size reached about 50 mm3, docetaxel was given through intraperitoneal injection with a dose of 1 mg/kg, one dose every other day with 3 doses in total. A. Growth curve of tumor volumes and representative photographs of tumors formed 35 days after the first administration of docetaxel. B. Quantification of E2F3a/b protein in the transplanted tumor tissues by Western Blot analysis. C. Quantification of miR-200b in the transplanted tumor tissues by qRT-PCR. Abundance of protein and miRNA was normalized to GAPDH and U6 RNA, respectively. D. Immunostaining of E2F3, Ki-67 and PCNA protein and TUNEL assay stained sections of the transplanted tumors as indicated (original magnification, ×400). *p < 0.05, **p < 0.01 vs. control group.