Skip to main content
. 2016 Mar 30;7(19):27787–27801. doi: 10.18632/oncotarget.8497

Figure 4. Mnk1 inhibitor CGP57380 abrogates the eIF4E phosphorylation induced by mTOR inhibitor RAD001 in vitro.

Figure 4

A. Western blotting analysis for the expression of several proteins including the phosphorylated and total levels of AKT, S6 and eIF4E in six kinds of human NSCLC cell lines. α-tubulin was used as a loading control. B. A549 cells were treated with the indicated concentrations of RAD001 (RAD) for 24 h and 48 h. C. A549 and H157 cells were treated with 5nM RAD001 (RAD) for the indicated times. D. H358 cells were treated with the indicated concentrations of CGP57380 (CGP) for 24 h. E. A549 cells were treated with combination of 5nM RAD001 (RAD) and the indicated concentrations of CGP57380 (CGP) for 24 h. F. A549 cells were treated with 5nM RAD001 (RAD) for 24 h and then co-treated with DMSO, or 10μM CGP57380 (CGP) for the indicated times. After these treatments, the cells were harvested for preparation of whole-cell protein lysates and subsequent Western blotting analysis to detect the indicated proteins. α-tubulin or GAPDH was used as a loading control.