Figure 5. Concomitant treatment with mTOR inhibitor and Mnk inhibitor attenuate eIF4E and AKT phosphorylation both in vitro and in vivo.

A. Cell lysates were collected from A549 and H157 cells which were treated with 25 nM RAD001 (RAD) alone, 25μM CGP57380 (CGP) alone and both combination for 24 h and subjected to Western blotting for the indicated proteins. GAPDH served as a loading control. B. IHC for p-AKT, p-S6 and p-eIF4E in tumor tissues from nude mouse xenograft models of human lung cancer cells.