Table 2. Differentially expressed protein spots in hippocampus between EE-treated TRPC1^−/− mice and non-treated TRPC1^−/− mice identified via 2D-DIGE/MALDI-TOF-MS/MS.

Spot No	Protein ID. a	Accession No.	Protein name b	Mascot score	Cove. c (%)	MWd (Da)	PI e	TRPC1−/−+EE vs TRPC1−/−
								Ratiof	P-value
513	5	G3P_MOUSE	Glyceraldehyde-3-phosphate dehydrogenase	117	4	36072	8.44	1.54	0.0056
514	6	G3P_MOUSE	Glyceraldehyde-3-phosphate dehydrogenase	120	4	36072	8.44	1.64	0.0065
20	7	ATPB_MOUSE	ATP synthase subunit beta, mitochondrial	254	10	56265	5.19	−1.5	0.0074
106	8	DPYL2_MOUSE	Dihydropyrimidinase-related protein 2	694	17	62638	5.95	−1.1	0.0089
61	9	NSF_MOUSE	Vesicle-fusing ATPase	170	6	83131	6.52	−1.3	0.0091
70	10	ACTBL_MOUSE	Beta-actin-like protein 2	74	10	42319	5.3	−1.2	0.0092
59	11	DPYL2_MOUSE	Dihydropyrimidinase-related protein 2	486	12	62638	5.95	−1.2	0.015
124	14	AINX_MOUSE	Alpha-internexin	91	12	55879	5.23	−1.3	0.017
110	13	PAK1_MOUSE	Serine/threonine-protein kinase PAK 1	51	3	61041	5.53	−1.3	0.017
115	16	DPYL2_MOUSE	Dihydropyrimidinase-related protein 2	617	15	62638	5.95	−1.1	0.022
527	33	GMFB_MOUSE	Glia maturation factor beta	137	42	16884	5.08	−1.4	0.023
30	17	ACON_MOUSE	Aconitate hydratase, mitochondrial	844	17	86151	8.08	−1.2	0.026
177	19	COR1A_MOUSE	Coronin-1A	234	11	51641	6.05	−1.3	0.028
113	18	GLSK_MOUSE	Glutaminase kidney isoform, mitochondrial	235	5	74771	8.23	−1.1	0.028
83	20	VATA_MOUSE	V-type proton ATPase catalytic subunit A	779	21	68625	5.42	−1.2	0.029
32	21	ACON_MOUSE	Aconitate hydratase, mitochondrial	933	17	86151	8.08	−1.2	0.03
90	24	ODP2_MOUSE	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial	811	15	68469	8.81	−1.2	0.033
91	27	GRP75_MOUSE	Stress-70 protein, mitochondrial	213	6	73768	5.91	−1.2	0.038
160	28	TCPB_MOUSE	T-complex protein 1 subunit beta	88	4	57783	5.97	−1.1	0.04
448	30	HPRT_MOUSE	Hypoxanthine-guanine phosphoribosyltransferase	134	9	24783	6.21	−1.1	0.041
74	31	VATA_MOUSE	V-type proton ATPase catalytic subunit A	345	11	68625	5.42	−1.2	0.042
129	32	CH60_MOUSE	60 kDa heat shock protein, mitochondrial	714	17	61088	5.91	−1.2	0.05

^aProtein ID assigned manually.

^bProtein name identified by MS.

^cSequence coverage achieved by MALDI-TOF-MS.

^dTheoretical molecular weight of the protein(s).

^eTheoretical isoelectrical point of the protein(s).

^fThe ratio in spot density from EE-treated TRPC1^−/− mice compared to TRPC1^−/− mice. n = 6 for both group.