Table 3. Differentially expressed protein spots in between EE treated-WT mice and non-treated WT mice identified via 2D-DIGE/MALDI-TOF-MS/MS.
| Spot No | Protein ID. a | Accession No. | Protein name b | Mascot score | Cove. c (%) |
MWd (Da) |
PI e | WT+EE vs WT | |
|---|---|---|---|---|---|---|---|---|---|
| Ratiof | P-value | ||||||||
| 93 | 1 | ODP2_MOUSE | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial | 383 | 9 | 68469 | 8.81 | −1.1 | 0.015 |
| 281 | 2 | PRS7_MOUSE | 26S protease regulatory subunit 7 | 124 | 4 | 49016 | 5.72 | 1.29 | 0.025 |
| 151 | 4 | DPYL2_MOUSE | Dihydropyrimidinase-related protein 2 | 460 | 13 | 62638 | 5.95 | 1.27 | 0.04 |
a
Protein ID assigned manually.
b
Protein name identified by MS.
c
Sequence coverage achieved by MALDI-TOF-MS.
d
Theoretical molecular weight of the protein(s).
e
Theoretical isoelectrical point of the protein(s).
f
The ratio in spot density from EE-treated WT mice compared to WT mice. n = 6 for both group.