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. 2016 Apr 4;7(19):27975–27987. doi: 10.18632/oncotarget.8564

Figure 2. KLF6 directly binds to the Sp1 and basigin-2 promoter.

Figure 2

A. The dual-luciferase reporter assay was performed by co-transfecting reporter vectors inserted with the basigin-2 promoter (basigin-2P/pGL3) or the Sp1 minimal promoter (Sp1-P/pGL3) with overexpression vectors or knockdown siRNA of KLF6 and Sp1, respectively. *, P < 0.05, using Student's t test. B. The ChIP assay demonstrated endogenous KLF6 and Sp1 binds to the basigin-2 and Sp1 promoter. The histograms represent quantification of ChIP results. C. Reporter assay results in cells transfected with various Sp1 and basigin-2 promoter constructs with mutations in KLF6 binding elements. Mu, mutation type. Luciferase activity was expressed as relative to that of the pGL3 vector. *, P < 0.05, using Student's t test.