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. 2016 Mar 30;7(19):28013–28026. doi: 10.18632/oncotarget.8472

Figure 4. HBx activates C4BPα promoter through transcription factor Sp1.

Figure 4

A. The illustration of the responsive region to HBx in C4BPα promoter was shown. B. The relative activities of the core promoter (−1199/−803nt) of C4BPα containing wild type (WT) or mutant type (MUT) of Sp1 binding site were detected by luciferase reporter gene assays in HepG2-X or (H7402-X) cells. C. The relative activities of the core promoter (−1199/−803nt) of C4BPα were detected by luciferase reporter gene assays in HepG2-X or (H7402-X) cells transiently transfected with Sp1 siRNA. D. The protein levels of C4BPα were examined by ELISA in the supernatant of HepG2-X (or H7402-X) cells treated with Sp1 siRNA. E. The interaction between HBx and Sp1 in HepG2-X cells was examined by ChIP assays. *p<0.05, **p<0.01, Student's t-test. All experiments were performed at least three times.