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. 2016 Mar 30;7(19):28013–28026. doi: 10.18632/oncotarget.8472

Figure 5. Down-regulation of C4BPα increases the sensitivity of hepatoma cells to CDC.

Figure 5

A. The efficiency of C4BPα siRNA was examined by ELISA in the supernatant of HepG2-X cells. B, C. The effect of C4BPα siRNA on CDC was determined by Trypan blue absorbance assays in HepG2, HepG2-X (or H7402, H7402-X) cells treated with different dilutions of normal human serum. D. The effect of antibody against C4BPα on CDC was examined by Trypan blue absorbance assay in HepG2, HepG2-X (or H7402, H7402-X) cells treated with 45% of normal human serum. E. The deposition of C5b-9 was measured by ELISA in HepG2, HepG2-X (or H7402, H7402-X) cells treated with C4BPα siRNA. F. The effect of Sp1 siRNA or overexpression of C4BPα on CDC was examined by Trypan blue absorbance assays in HepG2-X (or H7402-X) cells treated with 45% of normal human serum. *p<0.05, **p<0.01, Student's t-test. All experiments were performed at least three times.