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. 2016 Apr 4;7(19):28040–28058. doi: 10.18632/oncotarget.8575

Figure 3. Apoptotic and inflammatory responses in the hippocampus after irradiation at 10D or 10W.

Figure 3

A. and C. Expression and B. and D. quantification of cleaved caspase 3 immunostaining in specific regions (dashed lines) showing a significant increase in apoptosis after 10D- (B), but not 10W-irradiation with 2 Gy (D). Images, 10x magnification, scale bar = 100 μm; high magnification 100x, scale bar = 10 μm. E. and G. Representative immunostaining for Iba1 in 10D- and 10W-irradiated mice. Quantification of the expression level of Iba1 at 1 day F–H. after irradiation with 2 Gy of X-rays at 10D or 10W, showing a significant increase in Iba1 expression only in 10D-irradiated mice at 24 h after exposure. I. and K. Representative images of GFAP immunostaining at 6 months postirradiation. J. Significant increase in the number of GFAP+ astrocytes in non neurogenic areas (H and ML) of the hippocampus in 10D-irradiated mice. L. No changes were detected in 10W-irradiated mice. Image magnification 100x, scale bar =10 μm. Data are reported as mean ± SEM *P < 0.05; **P < 0.01; comparison with controls. The number of animals used is n = 4–5.