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. 2016 Apr 4;7(19):28040–28058. doi: 10.18632/oncotarget.8575

Figure 4. Proteomic analysis and immunoblotting validation of 10D and 10W irradiated mice at 6 months post-irradiation.

Figure 4

A. Venn diagram of all deregulated and shared proteins from global mass spectrometry-based protein quantification in hippocampus of mice irradiated with 2 Gy of X-ray at 10D and 10W. The number outside each circle shows the total number of deregulated proteins. B. Immunoblot analysis of postsynaptic protein PSD-95 (Dlg4), Rac1, Hspd1, and Sod1 in protein extracted from 10D- and 10W-irradiated hippocampus. C. Graphic representation of densitometric immunoblot analysis in control and irradiated samples. Normalization was performed against alpha-tubulin (Tub) as a loading control (n = 3, unpaired Student's t test, *P < 0.05, **P < 0.01). D. Signaling pathway analysis using Ingenuity Pathway Analysis software. High color intensity represents high significance (P-value). All colored boxes have a P value of ≤0.05; white boxes have a P value of ≥0.05. E. Protein-protein interaction network of the 10D and F. 10W exposed mice analyzed with the STRING software. The number of animals used for proteomic analysis is n = 4.